ProjectMechanism of Environmental Stress-Induced Developmental Abnormalities
Project Leader: Dennis Thiele
Publications ↓Project Description
Work in the Thiele laboratory focuses on copper-regulated transcription in Drosophila melanogaster, the identification of transcriptional responses to copper on a genome-wide level and the function of copper transport proteins in normal growth and development during copper deficiency and copper excess. These specific aims are as follows:
1. Understand how Drosophila MTF-1 both activates and represses gene transcription in response to the same signal
2. Understand how changes in copper levels cause specific stages of developmental arrest
3. Understand how changes in copper levels cause specific stages of developmental arrest
4. Identify new genes that respond to copper that may have conserved counterparts in fish, nematodes, mammals
Description ↑Publications
Song, MO, Li, J, Freedman, JH. 2009. Physiological and toxicological transcriptome changes in HepG2 cells exposed to copper. Physiol. Genomics 38, 386-401. PMCID 19549813
Mattie, M.D., McElwee, M., and Freedman, J.H. 2008. tivation of proto-oncogene expression and AP-1 activity through JNK/SAPK and p38 signaling pathways by copper-induced oxidative stress. J. Mol. Biol. 383:1008-1018.
Turski, M. L. and Thiele, D. J. 2007. Drosophila Ctr1A functions as a copper transporter essential for development. J. Biol. Chem. 282: 24017-24026.
Link: http://www.jbc.org/cgi/content/full/282/33/24017
Turski, M.L. and Thiele, D.J. 2007. Basic research on model systems: essential to understanding the causes and treatments for Wilson’s Disease and other disorders of copper metabolism. Wilson’s Disease Association International Newsletter.
Song, Min Ok and Jonathan H. Freedman. 2005. Activation of Mitogen Activated Protein Kinases by PCB126 (3,3,4.4,5-Pentachlorobiphenyl) in HepG2 Cells. Toxicological Sciences. 84(2):308-318.
Mattie, Michael D. and Jonathan H. Freedman. 2002. Copper-inducible transcription: regulation by metal- and oxidative stress-responsive pathways. American Journal of Physiology-Cell Physiology. 286(2):C293-C301.
Cioci, L.K., Ling Qui, and Jonathan H. Freedman. 2000. Transgenic strains of the nematode caenorhabditis elegans as biomonitors metal contamination. Environmental Toxicology and Chemistry. 19(8):2122-2129.
Tcherepanova, Irina, Lokesh Bhattacharyya, Charles S. Rubin, and Jonathan H. Freedman. 2000. Aspartic Proteases from the Nematode Caenorhabditis elegans. Structural Organization and Developmental and Cell-Specific Expression of asp-1. Journal of Biological Chemistry. 275(34):26359-26369.